Product Specific
HER-2/neu ELISA
Can urine samples be run on the HER-2/neu ELISA?
Urine samples are not validated for use in the HER-2/neu ELISA.
Can plasma samples be run on the HER-2/neu ELISA?
The intended use of the HER-2/neu ELISA for clinical purposes is for measurement of HER-2/neu protein in serum samples when monitoring patients with metastatic breast cancer.
Does the HER-2/neu ELISA measure phosphorylated or non-phosphorylated versions of the HER-2/neu oncoprotein?
The HER-2/neu ELISA reagents are directed to the p105 extracellular domain (ECD) of the HER-2/neu oncoprotein, and are not able to distinguish phosphorylation states of the full length molecule.
Can I run an overnight HER-2/neu assay?
No, the HER-2/neu ELISA IFU describe a one-day procedure only.
EGFr ELISA
What is measured in the EGFr ELISA?
The EGFr ELISA reagents are directed to the p110 extra cellular domain (ECD) of the EGFr oncoprotein.
Does the HER-2/neu p105 interfere with the EGFr ELISA?
The EGFr ELISA does not detect HER-2/neu p105. The EGFr ELISA has been challenged with concentrations of HER-2/neu that are ten-fold higher than the upper end of the test dynamic range with no interference detected.
What is the stability of EGFr in frozen sera, which has already been defrosted once?
Our evaluation of freeze/thaw stability of EGFr p110 indicates that the molecule is stable through at least six freeze-thaw cycles.
What are the expected values for the EGFr ELISA?
Refer to the Sample Values section in the EGFr ELISA Instructions for Use. The levels listed in the IFU should be used as a guideline only. The determination of expected values should be carried out by each laboratory using appropriate samples.
uPA ELISA
What are the expected values for the uPA ELISA?
Refer to the Sample Values section in the uPA ELISA IFU. The levels listed in the IFU should be used as a guideline only. The determination of expected values should be carried out by each laboratory using appropriate samples.
PAI-1 ELISA
What are the expected values for the PAI-1 ELISA?
Refer to the Sample Values section in the PAI-1 ELISA IFU. The levels listed in the IFU should be used as a guideline only. The determination of expected values should be carried out by each laboratory using appropriate samples.
Has Oncogene Science performed any comparison studies on what kind of plasma is most suited for the PAI-1 ELISA?
In comparative in-house PAI-1 studies, no differences were seen in results obtained from plasma samples under Heparin, EDTA, or Citrate sample collection conditions. Therefore, the customer should have confidence in their PAI-1 ELISA results no matter what anticoagulant/preservative is used with the plasma samples.
TIMP-1 ELISA
What is measured in the TIMP-1 ELISA?
The TIMP-1 ELISA quantifies total (free and complexed) tissue inhibitor of metalloproteinase-1 (TIMP-1).
What are the expected values for the TIMP-1 ELISA?
Refer to the Sample Values section in the TIMP-1 ELISA IFU. The levels listed in the IFU should be used as a guideline only. The determination of expected values should be carried out by each laboratory using appropriate samples.
CA IX ELISA
What is measured in the CA IX ELISA?
The CA IX ELISA is an enzyme-linked immunoassay that quantifies MN protein, also known as CA IX (carbonic anhydrase IX), in human serum and plasma.
What are the expected values for the CA IX ELISA?
Refer to the Sample Values section in the CA IX IFU. The levels listed in the IFU should be used as a guideline only. The determination of expected values should be carried out by each laboratory using appropriate samples.
Can you use any other type of rotating platform for incubating the CA IX ELISA?
No. You must use a titer plate shaker as described in the IFU.
